Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

10.1K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.1K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

4.0K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
4.0K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

14.2K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
14.2K
ER Retrieval Pathway01:45

ER Retrieval Pathway

4.7K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
4.7K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

16.8K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
16.8K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.9K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bi-directional regulation between NAD/NAMPT and IFN-γ/PD-L1 axes via BRD4/IRF1 and mitochondrial respiration in metastatic cutaneous melanoma.

Journal of experimental & clinical cancer research : CR·2026
Same author

Biophysical and aerodynamic properties of a peptide targeting the A-kinase anchoring function of PI3Kγ for pulmonary cAMP modulation.

Scientific reports·2026
Same author

Targeting PI3Kγ anchoring enhances CFTR membrane localization and modulator efficacy via PKD1.

JCI insight·2026
Same author

SH3BP5L triggers the RAB11A-regulated integrin recycling network implicated in breast cancer metastasis.

The Journal of clinical investigation·2026
Same author

Caught in the crossfire: cardiac complications of cancer therapy.

The Journal of clinical investigation·2026
Same author

Inhibition of HuR/ELAVL-1 attenuates fibrotic progression in Mdx mice with dilated cardiomyopathy.

Cellular and molecular life sciences : CMLS·2025
Same journal

Avoidance of immune mediated tumor rejection by cancer cells.

Advances in biological regulation·2026
Same journal

Advances in understanding the roles of TP53 mutations in pancreatic and hepatobiliary cancers.

Advances in biological regulation·2026
Same journal

Lipid-NLRP3 interplay in inflammasome regulation.

Advances in biological regulation·2026
Same journal

Modulation of skin inflammation by a recombinant Brown spider venom phospholipase D: In silico and in vitro approaches.

Advances in biological regulation·2026
Same journal

Adrenergic receptor activation shapes circadian clock gene oscillations in HL-1 cardiomyocyte cell line.

Advances in biological regulation·2026
Same journal

Ecnomotopic expression of olfactory receptors is affected by human apolipoproteins A-I<sub>Milano</sub> and A-II: evidence from liver microarray analyses.

Advances in biological regulation·2026
See all related articles

Related Experiment Video

Updated: Jan 13, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.6K

The interplay between phosphoinositides and ESCRT proteins.

Chiara Giannini1, Luca Ponzone1, Nicola Barroero1

  • 1Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Centre "Guido Tarone", University of Turin, Via Nizza 52, 10126, Turin, Italy.

Advances in Biological Regulation
|October 28, 2025
PubMed
Summary
This summary is machine-generated.

The Endosomal Sorting Complex Required for Transport (ESCRT) machinery uses phosphoinositides to remodel and repair cell membranes. This review details how specific phosphoinositide pools control ESCRT complex activity and recruitment.

More Related Videos

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

10.0K

Related Experiment Videos

Last Updated: Jan 13, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.6K
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

10.0K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The Endosomal Sorting Complex Required for Transport (ESCRT) machinery is crucial for various membrane remodeling and repair processes.
  • ESCRT complexes assemble hierarchically to detect, remodel, and sculpt cellular membranes with high precision.
  • Phosphoinositides (PtdIns) are key regulators of ESCRT function, influencing membrane identity and signaling.

Purpose of the Study:

  • To synthesize current understanding of phosphoinositide regulation of ESCRT machinery.
  • To elucidate the molecular mechanisms by which distinct phosphoinositide pools govern ESCRT recruitment and activity.

Main Methods:

  • Literature review and synthesis of existing research.
  • Analysis of molecular components and their interactions within the ESCRT pathway.
  • Focus on the role of phosphoinositides in membrane remodeling events.

Main Results:

  • Phosphoinositides act as central determinants of ESCRT function.
  • Specific phosphoinositide pools define membrane identity and recruit ESCRT modules.
  • Lipid signaling integrates into biological processes through ESCRT-mediated membrane dynamics.

Conclusions:

  • Distinct phosphoinositide pools are critical for precise control of ESCRT-mediated membrane remodeling and repair.
  • Understanding these lipid-protein interactions is key to deciphering cellular membrane dynamics.