Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Impact and Reliability of Tissue Segmentation on In Vivo Magnetic Resonance Spectroscopy Metabolite Quantification.

Magnetic resonance in medicine·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Alzheimer's Imaging Consortium.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Concerted elevations of cortical and striatal glutamate and GABA in antipsychotic-free individuals at clinical high-risk for psychosis.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2025
Same author

Modulation of neurometabolites in sensory processing brain areas during motor learning.

The Journal of physiology·2025
Same author

Benchmarking the Impact of Anatomical Segmentation on In Vivo Magnetic Resonance Spectroscopy.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 16, 2026

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism
13:56

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism

Published on: November 19, 2014

20.6K

Test-Retest Reliability of Multi-Metabolite Edited MRS at 3T Using PRESS and sLASER.

Jessica Archibald1, Amy E Bouchard1, Ralph Noeske2

  • 1Department of Radiology, Weill Cornell Medicine, New York, New York, USA.

Magnetic Resonance in Medicine
|October 28, 2025
PubMed
Summary

Spectral editing methods like HERMES and HERCULES offer reliable in vivo brain metabolite measurement. The sLASER localization technique demonstrated superior test-retest reliability compared to PRESS for most metabolites, enhancing measurement accuracy.

Keywords:
GABAglutathionemagnetic resonance spectroscopymetabolitesreliabilityspectral editing

More Related Videos

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease
09:30

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Published on: December 18, 2016

20.1K
Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner
08:36

Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner

Published on: June 7, 2024

642

Related Experiment Videos

Last Updated: Jun 16, 2026

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism
13:56

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism

Published on: November 19, 2014

20.6K
Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease
09:30

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Published on: December 18, 2016

20.1K
Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner
08:36

Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner

Published on: June 7, 2024

642

Area of Science:

  • Neuroimaging
  • Magnetic Resonance Spectroscopy (MRS)
  • Metabolomics

Background:

  • Spectral editing is crucial for noninvasive in vivo brain metabolite quantification, particularly for low-concentration, overlapped metabolites like γ-aminobutyric acid (GABA) and glutathione (GSH).
  • Advanced multi-metabolite editing techniques, including HERMES and HERCULES, allow simultaneous editing of multiple J-coupled metabolites without extending scan duration.
  • The test-retest reliability of these advanced multi-metabolite editing methods, especially concerning different volume localization techniques, remains underexplored.

Purpose of the Study:

  • To assess and compare the test-retest reliability of HERMES and HERCULES multi-metabolite editing sequences.
  • To evaluate the impact of two volume localization methods, PRESS and sLASER, on the reliability of these MRS sequences.
  • To determine which combination of editing sequence and localization method provides the most reliable in vivo quantification of brain metabolites.

Main Methods:

  • Sixteen healthy adults underwent two separate scanning sessions for test-retest reliability assessment.
  • Single-voxel edited MRS data were acquired from the medial parietal lobe using four sequences: HERMES-PRESS, HERMES-sLASER, HERCULES-PRESS, and HERCULES-sLASER.
  • Spectra were processed, and metabolite concentrations were quantified relative to total creatine (tCr), with data quality and reliability statistics calculated.

Main Results:

  • HERMES-sLASER exhibited lower within-subject coefficients of variation (CVws) for GABA+, GSH, glutamine (Gln), myo-inositol (mI), NAA, and total Cho (tCho) compared to HERMES-PRESS.
  • HERCULES-sLASER demonstrated improved reliability over HERCULES-PRESS for GABA+, GSH, aspartate, Gln, lactate, mI, NAA, and tCho, indicated by lower CVws.
  • While sLASER generally improved reliability, N-acetylaspartylglutamate (NAAG) showed higher variability with HERCULES-sLASER, and glutamate (Glu) with both HERMES- and HERCULES-sLASER.

Conclusions:

  • The sLASER localization technique provides superior test-retest reliability compared to PRESS for a majority of metabolites when used with multi-metabolite edited MRS sequences (HERMES and HERCULES).
  • These findings support the use of sLASER in conjunction with HERMES or HERCULES for more reliable in vivo brain metabolite measurements.
  • The choice of localization method significantly impacts the reliability of quantitative MRS, with sLASER emerging as a preferred option for enhanced measurement consistency.