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Updated: Jan 13, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

Specific microRNAs for Heart Failure: Reference Values in Whole Blood.

Radka Sigutova^1,2,3, Lukas Evin^4,5, Pavlina Kusnierova^1,2

¹Institute of Laboratory Medicine, Department of Clinical Biochemistry, University Hospital Ostrava, 708 52 Ostrava, Czech Republic.

|October 29, 2025

Summary

This summary is machine-generated.

This study established reference values for eight microRNAs (miRNAs) crucial in heart failure. These validated miRNA reference intervals provide essential benchmarks for future clinical research and diagnostics.

Keywords:

heart failure miRNA (microRNA)microRNA enzymatic immunoassay (miREIA) method reference values

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Area of Science:

Biochemistry
Molecular Biology
Cardiovascular Research

Background:

MicroRNAs (miRNAs) play a significant role in the pathophysiology of heart failure.
Establishing validated reference values for specific miRNAs is crucial for their clinical application.
Eight key miRNAs (hsa-miR-21-5p, hsa-miR-23a-3p, hsa-miR-142-5p, hsa-miR-126-3p, hsa-miR-499a-5p, hsa-miR-195-5p, hsa-miR-1-3p, hsa-miR-29a-3p) were selected due to their association with heart failure.

Purpose of the Study:

To validate reference values for eight selected microRNAs (miRNAs) in a healthy adult population.
To determine reference intervals for these miRNAs in whole blood samples.
To investigate potential influences of age and sex on miRNA reference values.

Main Methods:

Ninety-nine healthy individuals were enrolled in the study.
MicroRNAs were isolated from whole blood and quantified using the microRNA enzymatic immunoassay (miREIA) method.
Statistical analyses were performed using MedCalc and R software to determine reference intervals (2.5th and 97.5th percentiles) and evaluate age/sex associations.

Main Results:

Reference intervals were established for all eight miRNAs, with values provided for each (e.g., hsa-miR-21-5p: 1.45-96.3 pmol/L).
No significant sex-related differences were observed in the reference intervals (p < 0.05).
A significant age-related association was found only for hsa-miR-21-5p (RS = -0.208; p = 0.043); other miRNAs showed no significant age correlation (p < 0.05).

Conclusions:

Validated reference intervals for eight heart failure-associated miRNAs in healthy individuals were successfully determined.
These absolute reference values are novel and essential for future clinical research and diagnostic applications.
While age and sex were evaluated, reference intervals were not stratified due to subgroup size limitations, highlighting a need for larger cohort studies.