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Related Concept Videos

Gene Therapy00:59

Gene Therapy

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.3K

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Related Experiment Video

Updated: Jan 13, 2026

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

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Surgical Approaches to Retinal Gene Therapy: 2025 Update.

Milin J Patel1,2, Sohum Sheth3, Jessica Mar1

  • 1Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.

Bioengineering (Basel, Switzerland)
|October 29, 2025
PubMed
Summary
This summary is machine-generated.

Gene therapy is a promising treatment for retinal diseases. This review covers current surgical delivery methods for gene therapy to the retina, including subretinal, suprachoroidal, and intravitreal approaches, updated for 2025.

Keywords:
age-related macular degenerationgene therapyinherited retinal diseaseintravitrealretinasubretinalsuprachoroidal

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Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
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Last Updated: Jan 13, 2026

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Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Inherited and acquired retinal diseases represent a significant unmet medical need.
  • Gene therapy has emerged as a potent therapeutic strategy for restoring vision.
  • Effective delivery of gene therapy vectors to the retina is crucial for successful treatment.

Purpose of the Study:

  • To review and update the state of the art in surgical gene therapy delivery to the retina in 2025.
  • To compare the advantages and disadvantages of different retinal gene therapy delivery methods.
  • To inform future research and clinical applications in retinal gene therapy.

Main Methods:

  • Review of current literature on retinal gene therapy delivery techniques.
  • Analysis of surgical approaches including subretinal, suprachoroidal, and intravitreal injections.
  • Evaluation of preclinical and clinical data for each delivery method.

Main Results:

  • Subretinal delivery remains a primary method, offering direct access to photoreceptors.
  • Suprachoroidal injection provides a less invasive option with potential for broader retinal coverage.
  • Intravitreal delivery is the least invasive but faces challenges with efficient retinal transduction.

Conclusions:

  • Each surgical delivery method for retinal gene therapy has unique benefits and limitations.
  • Advancements in surgical techniques continue to improve the safety and efficacy of retinal gene therapy.
  • The choice of delivery method depends on the specific retinal disease and target cell population.