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Related Experiment Video

Updated: Jan 13, 2026

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
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Characterization of Corneal Defects in ATG7-Deficient Mice.

Thomas Volatier1, Andreas Mourier1, Johanna Mann1

  • 1Department of Ophthalmology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50923 Köln, Germany.

International Journal of Molecular Sciences
|October 29, 2025
PubMed
Summary

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Early postoperative corneal thickness and 12-month endothelial cell outcomes after descemet membrane endothelial keratoplasty: A multicentre analysis.

Acta ophthalmologica·2026
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Living tissues and dead objects in the eye: The first description of ocular immune privilege by J.C. van Dooremaal in Albrecht Von Graefe's Archiv für Ophthalmologie in 1873.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
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Corneal transplantation: Restoring sight to the blind.

Acta ophthalmologica·2026
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Limbal Epithelial Stem Cells in Review: Immune and Lymphangiogenic Privilege and Their Clinical Relevance.

Cells·2026
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Higher-Order Aberrations, Corneal Density, and Visual Acuity After Excimer Laser Phototherapeutic Keratectomy (PTK) for Epithelial Basement Membrane Dystrophy.

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Furin-like cleavage at the C1-C2 linker region of the ⍺3 chain is not required for collagen VI assembly.

Matrix biology : journal of the International Society for Matrix Biology·2025

Autophagy gene 7 (ATG7) is crucial for maintaining corneal epithelial health. ATG7 deficiency disrupts autophagy, leading to corneal thickening and increased lymphatic vessel growth, impacting ocular surface disease.

Area of Science:

  • Cell Biology
  • Ophthalmology
  • Molecular Biology

Background:

  • Regulated proteolysis via autophagy is vital for cellular homeostasis.
  • The specific role of autophagy-related gene 7 (ATG7) in corneal epithelial maintenance is not well understood.

Purpose of the Study:

  • To investigate the impact of ATG7 deficiency on corneal epithelial autophagy, morphology, and vascular dynamics using a conditional knockout mouse model.

Main Methods:

  • Utilized a conditional knockout mouse model (Atg7(f/f) K14Cre(+/-)).
  • Assessed corneal epithelial autophagy, morphology, and vascular dynamics.
  • Performed proteomic analysis to identify compensatory pathways.

Main Results:

  • ATG7 deficiency disrupted autophagosome formation, indicated by altered LC3B expression and puncta.
Keywords:
ATG7autophagycorneacorneal epitheliumcorneal homeostasis

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  • Corneal epithelium thickened, and peripheral lymphatic vessel sprouting increased, suggesting a pro-inflammatory and pro-lymphangiogenic environment.
  • Proteomics revealed upregulation of RAB8, TM9S3, and RETR3, indicating compensatory pathways like exophagy, reticulophagy, and Golgiphagy.

    • Conclusions:

    • ATG7-mediated autophagy is essential for maintaining corneal epithelial homeostasis and immune privilege.
    • ATG7 deficiency leads to a pro-inflammatory and pro-lymphangiogenic microenvironment in the cornea.
    • Findings have implications for understanding corneal inflammation and lymphangiogenesis in ocular surface diseases.