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TaCML49-B, a Calmodulin-like Protein, Interacts with TaIQD23 to Positively Regulate Salt Tolerance in Wheat
Jingna Ru1, Jiamin Hao2, Bingqing Hao2
1The Industrial Crop Institute, Key Laboratory of Sustainable Dryland Agriculture of Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, China.
Plants (Basel, Switzerland)
|October 29, 2025
Summary
Wheat
Area of Science:
- Plant biology
- Molecular biology
- Biochemistry
Background:
- Calcium signaling regulates plant growth and stress responses.
- Calmodulin-like (CML) proteins in wheat are not fully understood.
- Many CML interacting partners and functions remain elusive.
Purpose of the Study:
- To characterize subgroup II CaM/CMLs in wheat using bioinformatics.
- To investigate the role of TaCML49-B in wheat salt stress response.
- To identify TaCML49-B interacting partners.
Main Methods:
- Bioinformatics analysis (phylogeny, motifs, cis-elements).
- Gene expression analysis (RT-qPCR).
- Subcellular localization, functional assays (Arabidopsis overexpression, wheat VIGS), protein-protein interaction (STRING, BiFC).
Main Results:
- TaCML49-B gene expression increased in wheat roots under salt stress.
- TaCML49-B localized to the nucleus, cytoplasm, and membrane.
- Overexpression of TaCML49-B enhanced salt tolerance in Arabidopsis, while its silencing reduced salt resistance in wheat.
- TaCML49-B interacts with TaIQD23, suggesting a role in salt stress signaling.
Conclusions:
- TaCML49-B plays a positive role in wheat salt stress response.
- This study provides new insights into TaCML gene function and calcium signaling in wheat.
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