The Effectiveness of plant-based Bioinsecticides in controlling Aedes aegypti larva populations: Scoping review

This study aimed to systematically review the effectiveness of plant-based bioinsecticides in controlling Aedes aegypti larvae, the primary vector of dengue, Zika, and chikungunya. It also sought to explore formulation innovations, identify implementation challenges, and propose directions for future research. A scoping review was conducted following the PRISMA guidelines to ensure comprehensive and structured analysis.

A literature search was performed in PubMed, ScienceDirect, and Scopus for peer-reviewed articles published between 2020 and 2025. Keywords related were used. Data extraction included study characteristics, plant species, compound type, formulation, concentration, and larvicidal outcomes.

Out of 1,142 identified records, 225 articles were assessed in full, and 21 studies met the final inclusion criteria. Results showed that plant-based bioinsecticides, particularly those using active compounds such as azadirachtin, citronella, and capsaicin, demonstrated significant larvicidal efficacy. Nanoparticle formulations and combinations of multiple plant extracts exhibited higher potency and faster larval mortality rates at lower concentrations.

Plant-based bioinsecticides present a promising eco-friendly alternative to synthetic insecticides. Effectiveness varies with extraction methods and formulation types. Modern approaches, such as nanoformulations and synergistic plant combinations, enhance larvicidal potential. Future studies should focus on optimizing formulation techniques and validating efficacy through field trials for sustainable vector control.