Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Background and Environment Affect Phenotype02:27

Background and Environment Affect Phenotype

7.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.4K
Osmoregulation in Insects01:47

Osmoregulation in Insects

17.5K
Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.
17.5K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome-level genome assembly of the Erythrina gall wasp, Quadrastichus erythrinae (Hymenoptera: Eulophidae).

G3 (Bethesda, Md.)·2026
Same author

An interdisciplinary approach to investigating an invasive insect pest: tracking, phenology, and genetics of the Queensland longhorn beetle, Acalolepta aesthetica (Cerambycidae: Lamiinae).

Journal of insect science (Online)·2026
Same author

A chromosome level reference genome for the pecan weevil, Curculio caryae.

Scientific data·2026
Same author

Haplotype-resolved genome assemblies for the New World screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), using the trio binning approach.

G3 (Bethesda, Md.)·2026
Same author

Reference genome assemblies for the North American bumble bees Bombus flavifrons and Bombus fervidus, two phenotypically polymorphic species from distinct phylogenetic lineages.

G3 (Bethesda, Md.)·2026
Same author

CiFi: accurate long-read chromosome conformation capture with low-input requirements.

Nature communications·2025

Related Experiment Video

Updated: Jan 13, 2026

Determining Temperature Preference of Mosquitoes and Other Ectotherms
05:31

Determining Temperature Preference of Mosquitoes and Other Ectotherms

Published on: September 28, 2022

2.7K

Symbiont Gene Expression Predicts Insect Host's Response to High Temperatures.

Patrick T Stillson1, Sheina B Sim2, Renee L Corpuz2

  • 1Department of Biology, University of Texas at Arlington, Arlington, Texas, USA.

Molecular Ecology
|October 29, 2025
PubMed
Summary

Host-symbiont interactions are key for survival, but heat stress can disrupt them. This study shows that symbiont gene expression, not host, determines bug performance under thermal stress, revealing context-dependent symbiosis outcomes.

Keywords:
Leptoglossus phyllopusCaballeroniaHeteropteragene expressionheat shocksymbiosisthermal stress

More Related Videos

High-Throughput Assays of Critical Thermal Limits in Insects
06:58

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

5.7K
Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
09:09

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Published on: December 30, 2014

8.4K

Related Experiment Videos

Last Updated: Jan 13, 2026

Determining Temperature Preference of Mosquitoes and Other Ectotherms
05:31

Determining Temperature Preference of Mosquitoes and Other Ectotherms

Published on: September 28, 2022

2.7K
High-Throughput Assays of Critical Thermal Limits in Insects
06:58

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

5.7K
Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
09:09

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Published on: December 30, 2014

8.4K

Area of Science:

  • Microbial Ecology
  • Symbiosis Research
  • Insect-Microbe Interactions

Background:

  • Microbial symbionts are vital for host health, providing nutrition, defense, and detoxification.
  • Environmental stressors, particularly thermal stress, can disrupt host-symbiont relationships, negatively impacting host performance.
  • Previous studies show thermal stress affects symbionts, leading to host vulnerability, but the mechanisms driving variation in host outcomes remain unclear.

Purpose of the Study:

  • To investigate the mechanisms underlying variable insect performance at elevated temperatures based on hosted symbiont species.
  • To compare host and symbiont transcriptional responses to thermal stress in a bug-Caballeronia model system.
  • To test if symbionts conferring better host outcomes under heat stress exhibit enhanced heat shock gene regulation.

Main Methods:

  • Comparative metatranscriptomics was employed to analyze gene expression in both host insects and their symbionts.
  • Two symbiont species with contrasting effects on host performance at high temperatures were utilized.
  • Transcriptional responses of both host and symbiont were evaluated under varying temperature conditions.

Main Results:

  • Host transcriptional patterns were primarily influenced by temperature, not by the specific symbiont species present.
  • Distinct gene expression profiles were observed between symbionts across different temperatures.
  • The heat-resistant symbiont upregulated heat shock genes and unexpectedly activated flagellar genes at 36°C, a departure from typical symbiotic gene expression.

Conclusions:

  • Symbiont transcriptional regulation, rather than host transcription, is the primary driver of host benefits under varying temperatures.
  • The findings highlight the critical role of symbiont responses in mediating host performance during environmental stress.
  • This research deepens the understanding of context-dependent outcomes in symbiotic associations and their resilience to climate change.