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Related Experiment Video

Updated: Jan 13, 2026

Thermal Imaging to Study Stress Non-invasively in Unrestrained Birds

Thermal Imaging to Study Stress Non-invasively in Unrestrained Birds

Published on: November 6, 2015

ForSys: Non-invasive stress inference from time-lapse microscopy.

Augusto Borges^1,2, Jerónimo R Miranda-Rodríguez^1,3, Alberto S Ceccarelli^4,5

¹Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich, Germany.

|October 29, 2025

Summary

This summary is machine-generated.

ForSys software estimates cell stresses during tissue development using microscopy. It reveals mechanical forces driving cell behavior and tissue patterns in living organisms.

Keywords:

Optical imaging

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Last Updated: Jan 13, 2026

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Area of Science:

Biophysics
Developmental Biology
Computational Biology

Background:

Mechanical forces are crucial for tissue development and regeneration.
Measuring these forces in vivo is challenging.
Existing stress inference algorithms struggle to incorporate tissue dynamics.

Purpose of the Study:

Introduce ForSys, a novel Python-based software for inferring intercellular stresses and intracellular pressures.
Enable dynamic stress inference from time-lapse microscopy data.
Analyze spatiotemporal patterns of mechanical forces during tissue morphogenesis.

Main Methods:

Developed ForSys, a versatile software tool.
Validated ForSys using simulated and experimental data.
Applied ForSys to zebrafish lateral-line primordium migration and neuromast development.
Integrated ForSys with Fiji for a graphical user interface and accepted segmentation from EPySeg and Cellpose.

Main Results:

ForSys successfully infers intercellular stresses and intracellular pressures from time-lapse microscopy.
Revealed increased stress during cell rounding preceding mitosis in zebrafish.
Accurately predicted the onset of epithelial rosettogenesis.
Uncovered mechanical asymmetries in neuromast development linked to cell type-specific adhesion.

Conclusions:

ForSys provides a powerful and versatile tool for analyzing mechanical forces in vivo.
The software facilitates the study of how mechanical forces regulate tissue morphogenesis and regeneration.
Enables dynamic stress inference, advancing the field of biophysical analysis in developmental biology.