Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multistimuli-Responsive Porous Organic Polymers for Drug-Gas Anticancer Combination Therapy.

ACS applied materials & interfaces·2025

Same author

Raman Spectroscopy and FRAP Analysis on Density Heterogeneity and Diffusion Behavior of Complex Coacervation by Arginine-Rich Dipeptides and Poly‑A RNA.

ACS omega·2025

Same author

A Sub-Diffraction-Limit Dimension All-Plasmonic Optical Memory Using Non-Linear Photochromism.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Remote Excitation of Tip-Enhanced Photoluminescence with a Parallel AgNW Coupler.

ACS omega·2023

Same author

A light-mediated covalently patterned graphene substrate for graphene-enhanced Raman scattering (GERS).

Chemical communications (Cambridge, England)·2023

Same author

Visualizing Ribbon-to-Ribbon Heterogeneity of Chemically Unzipped Wide Graphene Nanoribbons by Silver Nanowire-Based Tip-Enhanced Raman Scattering Microscopy.

Small (Weinheim an der Bergstrasse, Germany)·2023

Search research articles

Related Experiment Video

Updated: Jan 13, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Photocleavable Systems for Cell Biology: Conceptual Design across Molecular Modalities.

Masahiko Yoshimura¹, Tomoko Inose^1,2,3,4

¹Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Institute for Advanced Study, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.

Chembiochem : a European Journal of Chemical Biology

|October 29, 2025

Summary

Researchers review light-triggered bond cleavage systems for controlling biological functions. They classify proteins, small molecules, and metal complexes, discussing design, applications, and challenges for next-generation photo-responsive tools.

Keywords:

chemical biology coumarin photochemistry photocleavage photolysis

More Related Videos

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Related Experiment Videos

Last Updated: Jan 13, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Area of Science:

Chemical Biology
Cell Biology
Biomolecular Engineering

Background:

Spatiotemporal control of biomolecular functions is crucial for understanding biological processes.
Light-triggered bond cleavage offers precise external control over molecular events.
Photo-cleavable systems are increasingly vital tools in biological research.

Purpose of the Study:

To review and classify major modalities of photo-cleavable systems.
To highlight design principles, biological applicability, and challenges of these systems.
To provide perspectives on future developments in photo-responsive tools.

Main Methods:

Classification of photo-cleavable systems into proteins, small molecules, and metal complexes.
Discussion of design principles and biological applicability for each modality.
Analysis of recent efforts to address key design challenges.

Main Results:

Photo-cleavable systems are categorized based on their molecular modality.
Key design challenges include balancing performance, biocompatibility, and optical responsiveness.
Recent advancements aim to improve the efficacy and applicability of these tools.

Conclusions:

Photo-cleavable systems offer powerful spatiotemporal control in biology.
Addressing design challenges is key to advancing photo-responsive tools.
Future research will focus on next-generation tools for biological applications.