Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The bile acid-gut microbiota-vitamin D axis: new insights into biliary atresia.

Frontiers in cellular and infection microbiology·2026
Same author

Causal effects of endometriosis on serum 25-hydroxyvitamin D: Evidence from Mendelian randomization study.

Medicine·2026
Same author

Nanopore Discriminating d-Glucose Enantiomer Using a Designed Chiral Receptor.

Analytical chemistry·2026
Same author

Tunicamycin-induced endoplasmic reticulum stress triggers ZBP1-Mediated PANoptosis in macrophages and mouse spleen.

Molecular biology reports·2026
Same author

Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy.

Bioactive materials·2026
Same author

Dual Recognition and Highly Sensitive Detection of ATP by Aptamer-Bridged MOF Functionalized Glass Nanopipette.

Analytical chemistry·2026

Related Experiment Video

Updated: Jan 13, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
15:17

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

14.3K

Bubble Multimer-Enhanced Fluorescence Enables Self-Contained Profiling of Colorectal Cancer Extracellular Vesicles.

Yinghong Wei1,2, Binqi Wei2, Xifeng Mo2

  • 1Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

Nano Letters
|October 29, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel bubble-based assay for rapid and sensitive detection of tumor extracellular vesicles (tEVs) in blood. The technology enables accurate early diagnosis of colorectal cancer (CRC) using a simple blood test.

Keywords:
antifouling nanofilmbioorthogonal chemistrybubble-enhanced fluorescencecancer diagnosticsextracellular vesicles

More Related Videos

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

10.3K
Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

23.7K

Related Experiment Videos

Last Updated: Jan 13, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
15:17

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

14.3K
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

10.3K
Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

23.7K

Area of Science:

  • Biomarkers
  • Nanotechnology
  • Cancer Diagnostics

Background:

  • Tumor extracellular vesicles (tEVs) show promise as noninvasive biomarkers for cancer diagnosis due to their abnormal surface protein expression.
  • Current methods for isolating and profiling tEVs from biofluids are complex and challenging, hindering clinical application.

Purpose of the Study:

  • To develop a streamlined assay for rapid enrichment and sensitive profiling of plasma tEVs for accurate colorectal cancer (CRC) diagnosis.
  • To introduce a novel Lab-on-Bubbles technology for blood-based cancer detection.

Main Methods:

  • A bubble multimer-enhancing fluorescence (BMEF)-driven extracellular vesicles (BMEF-EV) assay was developed using multifunctional bubbles.
  • Bubbles were functionalized with antifouling nanofilm, clickable moieties, and antibodies for sequential capture and profiling of tEVs.
  • The assay leverages buoyant bubbles for spontaneous EV aggregation and a BMEF effect for sensitive detection.

Main Results:

  • The BMEF-EV assay successfully enabled rapid enrichment and sensitive profiling of plasma tEVs.
  • Profiling multiple CRC EV markers in a pilot cohort achieved near 90% accuracy for early CRC diagnosis.
  • The Lab-on-Bubbles technology demonstrated its potential as a self-contained tool for blood-based cancer diagnosis.

Conclusions:

  • The BMEF-EV assay offers a streamlined and sensitive approach for isolating and profiling tEVs.
  • This technology facilitates accurate and early diagnosis of colorectal cancer through a blood-based test.
  • The Lab-on-Bubbles platform presents a promising advancement for noninvasive cancer diagnostics.