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Updated: Jan 13, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

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Meteorin Is a Novel Interaction Partner of p60-Katanin During Mitosis in HCT-116 Colorectal Cancer Cells

Ilgin Isiltan1,2, Arzu Karabay1,2

  • 1Molecular Biology-Genetics and Biotechnology, Graduate School, Istanbul Technical University, Istanbul, Turkey.

Cytoskeleton (Hoboken, N.J.)
|October 29, 2025
PubMed

Insights

Meteorin interacts with p60-Katanin, a microtubule-severing protein, during cell division in colorectal cancer cells. Depleting Meteorin reduces cancer cell proliferation, highlighting its role in cell cycle progression.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Microtubule dynamics are crucial for cellular functions, regulated by proteins like p60-Katanin.
  • p60-Katanin is a microtubule-severing protein vital for cell division and microtubule remodeling.
  • Colorectal cancer (CRC) progression involves complex cellular mechanisms.

Purpose of the Study:

  • To identify novel interaction partners of p60-Katanin in colorectal cancer cells.
  • To investigate the role of Meteorin in cell division and proliferation of CRC cells.
  • To explore the relationship between Meteorin, p60-Katanin, and TP53 in CRC.

Main Methods:

  • Co-immunoprecipitation to identify protein interactions.
  • Immunofluorescence microscopy to determine protein localization during mitosis.
  • RNA interference (siRNA) to silence Meteorin expression.
  • Cell proliferation assays.

Main Results:

  • Meteorin was identified as a novel interaction partner of p60-Katanin in HCT-116 CRC cells.
  • Meteorin localizes to spindle poles throughout mitosis and co-localizes with p60-Katanin.
  • Meteorin silencing reduced HCT-116 and HCT-116 p53 (-/-) cell proliferation.
  • Meteorin levels influenced p60-Katanin expression, with silencing decreasing it and overexpression increasing it in TP53-expressing cells.

Conclusions:

  • Meteorin interacts with p60-Katanin and localizes to spindle poles during mitosis in CRC cells.
  • Meteorin depletion inhibits CRC cell proliferation independently of TP53 status.
  • Meteorin plays a significant role in regulating p60-Katanin expression and microtubule dynamics in colorectal cancer.
Keywords:
HCT 116meteorinmicrotubulemitosisp60‐kataninproliferation

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