Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A physics-informed alternative to Richardson-Lucy deconvolution across SNR regimes without iteration cutoffs.

Nature communications·2026

Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026

Same author

Stochastic colonization and host-to-host transmission shape gut bacterial variability.

bioRxiv : the preprint server for biology·2026

Same author

Simulation-based inference captures non-Markovian effects as exemplified in protein production kinetics through cell division.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Substrate-interacting pore loops of two ATPase subunits determine the degradation efficiency of the 26S proteasome.

Nature communications·2026

Same author

A cautious user's guide in applying HMMs to physical systems.

The Journal of chemical physics·2025

Same journal

Revisiting crossed-correlated baths in open quantum systems simulated by HEOM or T-TEDOPA.

The Journal of chemical physics·2026

Same journal

Vesicle size and membrane composition control monomer transfer pathways in multicomponent lipid vesicles.

The Journal of chemical physics·2026

Same journal

Polaron-mediated exciton dynamics of P(NDI2OD-T2) unveiled by transient absorption spectroscopy under electrochemical conditions.

The Journal of chemical physics·2026

Same journal

Green-Kubo relation in a mesoscale odd fluid model.

The Journal of chemical physics·2026

Same journal

Nitrogenation of microscopic MoS2 surfaces by oxidation scanning probe lithography.

The Journal of chemical physics·2026

Same journal

Molecular structure, binding, and disorder in TDBC-Ag plexcitonic assemblies.

The Journal of chemical physics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Perspective: An outlook on fluorescence tracking.

Lance W Q Xu^1,2, Steve Pressé^1,2,3

¹Center for Biological Physics, Arizona State University, Tempe, Arizona 85287, USA.

The Journal of Chemical Physics

|October 29, 2025

Summary

This summary is machine-generated.

Single-molecule tracking advances microscopy, revealing molecular dynamics. New methods enhance resolution and efficiency for future studies.

More Related Videos

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Related Experiment Videos

Last Updated: Jan 6, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

Biophysics
Microscopy
Molecular Biology

Background:

Single-molecule tracking provides insights into dynamic molecular processes.
Fluorescence microscopy is key to observing these processes at the single-molecule level.

Purpose of the Study:

To trace the evolution of single-molecule tracking techniques.
To highlight key methodological developments in fluorescence microscopy for tracking.
To explore future directions and challenges in the field.

Main Methods:

Review of conventional widefield offline tracking.
Analysis of real-time confocal tracking.
Exploration of physics-inspired tracking techniques.

Main Results:

Different tracking data yield varying insights into molecular behavior.
Advancements aim for higher spatiotemporal resolution.
Emerging techniques focus on computational and data efficiency.

Conclusions:

Single-molecule tracking has evolved significantly with microscopy advancements.
Future research will leverage parallelization and artificial intelligence.
Overcoming challenges will drive next-generation single-molecule studies.