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Fluorescence Sensor for pH Tracing Based on Hydrogen-Bonded Organic Frameworks.

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Researchers developed a pH-responsive fluorescent system using hydrogen-bonded organic frameworks (HOFs). This system shows promise for detecting histamine, protamine, and monitoring food spoilage through distinct fluorescence changes.

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Area of Science:

  • Materials Science
  • Chemistry

Background:

  • Hydrogen-bonded organic frameworks (HOFs) are advanced porous materials with tunable properties.
  • Organic ligands with acid-base sensitive groups are crucial for developing responsive materials.

Purpose of the Study:

  • To prepare pH-responsive HOFs (PFC-1) for a fluorescent sensing system.
  • To investigate the mechanism of fluorescence changes in response to pH variations.
  • To demonstrate the application of HOFs as pH indicators for biomolecule detection and food spoilage monitoring.

Main Methods:

  • Synthesis of HOFs (PFC-1) incorporating H4TBAPy ligands.
  • Characterization of fluorescence response to pH changes.
  • Application in detecting histamine and protamine concentrations.
  • Utilizing fluorescence changes to monitor food spoilage via biogenic amine detection.

Main Results:

  • The developed HOFs exhibit a facile pH-responsive fluorescent system.
  • Alkali addition leads to deprotonation, inhibiting ICT, reconstructing hydrogen bonds, widening the band gap, and enhancing the RIM effect.
  • This results in a blue shift and enhanced fluorescence emission.
  • The system successfully detected histamine and protamine and monitored food spoilage through distinct fluorescence changes.

Conclusions:

  • pH-responsive HOFs (PFC-1) offer a promising platform for fluorescent sensing.
  • The observed fluorescence changes are linked to molecular and electronic structure modifications.
  • This work lays a foundation for HOF applications in food safety, environmental monitoring, and biomedical sensing.