Exploring novel therapeutic strategies: Static Magnetic Fields in combination with doxorubicin induce ROS and
Amirsalar Nikkhah Bahrami1, Mohammad Hadi Sadeghian2, Nader Vazifeh Shiran3
1Department of Hematology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Abstract:
Acute lymphoblastic leukemia (ALL), primarily affecting children, is a rare malignancy. This study explores the use of alternative therapies such as Static Magnetic Fields (SMF) and Doxorubicin (Dox) as a novel therapeutic strategy for ALL NALM-6 cells. Viability, apoptosis, cell cycle, ROS, and gene expression were assessed using Trypan blue, MTT, flow cytometry, and qRT-PCR. SMF significantly reduced NALM-6 viability and metabolic activity, increased apoptosis and ROS, and altered gene expression related to apoptosis, ROS, cell cycle, and autophagy. Co-treatment with SMF and Dox enhanced these effects compared to single treatments (p ≤ .05), with no cytotoxicity to normal PBMCs or L929 cells. SMF alone or with Dox induces ROS-mediated apoptosis and autophagy inhibition, suggesting a potential complementary therapy, though further research is needed.
