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Updated: Jul 1, 2026
07:24
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Published on: December 10, 2014
14.9K
Genomic Insights into Poultry-Associated Staphylococcus Aureus from Haripur, Pakistan
Arif Ullah1, Bushra Fatima1, Mohammad Ejaz2
1Department of Microbiology, The University of Haripur, Haripur, Pakistan.
Current Microbiology
|October 29, 2025
Summary
This study reveals Staphylococcus aureus strains ST291 and ST88 in Pakistani poultry, carrying antibiotic resistance and virulence genes. Findings highlight risks of zoonotic spread and the need for better biosecurity and antibiotic stewardship.
Area of Science:
- Microbiology
- Genomics
- Veterinary Public Health
Background:
- Staphylococcus aureus poses risks in poultry due to antibiotic resistance and zoonotic potential.
- Poultry farming is a critical sector where pathogen surveillance is essential.
Purpose of the Study:
- To investigate the genetic diversity and antibiotic resistance of S. aureus in Pakistani poultry.
- To identify virulence factors and mobile genetic elements in S. aureus isolates.
Main Methods:
- Whole genome sequencing (WGS) of 24 S. aureus isolates from poultry samples.
- Standard microbiological isolation techniques.
- Genomic analysis for antibiotic resistance and virulence genes.
Main Results:
- Dominant sequence types (STs) identified were ST291 (n=18) and ST88 (n=6).
- Multiple antibiotic resistance genes (e.g., blaZ, gyrA, parC, tet, erm) and virulence genes (adhesion, biofilm, toxin, immune evasion) were detected.
- All isolates contained mobile genetic elements, indicating potential for gene spread.
Conclusions:
- Poultry in Pakistan harbor S. aureus with significant antibiotic resistance and virulence traits.
- The presence of mobile genetic elements facilitates the spread of resistance and virulence genes.
- Enhanced biosecurity and judicious antibiotic use are crucial to mitigate public health risks.
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