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POMPOMS: Crosslinked Biomolecular Condensates as a Versatile Platform for Multifunctional Protein Microparticles
Augene S Park1, Erika A Ding1, Benjamin S Schuster1
1Department of Chemical and Biochemical Engineering, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854, United States.
Biomacromolecules
|October 29, 2025
Summary
Researchers developed protein-based microparticles using biomolecular condensates. This new method allows for controlled size and functionalization, offering a versatile platform for biocatalysis and biomolecular capture applications.
Area of Science:
- Biomaterials Science
- Chemical Engineering
- Molecular Biology
Background:
- Protein-based microparticles offer potential for biocatalysis and biomolecular capture.
- Existing fabrication methods face challenges like protein denaturation and limited spatial control.
Purpose of the Study:
- To develop a novel method for synthesizing protein-based microparticles with controlled size and functionality.
- To overcome limitations of current techniques in protein material fabrication.
Main Methods:
- Utilized liquid-liquid phase separation of RGG domains to form protein droplets.
- Chemically crosslinked droplets using BS3 to create porous microparticles (POMPOMS).
- Modulated protein concentration and condensate coalescence to control microparticle size (<1 to >40 μm).
Main Results:
- Demonstrated tunable microparticle size by controlling protein concentration and coalescence.
- Engineered three functionalities: cargo protein capture via SpyCatcher/SpyTag, spatial organization in core-shell structures, and enzyme immobilization (31% retained activity).
Conclusions:
- Developed POMPOMS, a sustainable and tunable platform for versatile protein-based materials.
- The method enables precise control over microparticle size and spatial organization.
- Showcased potential applications in biocatalysis and biomolecular capture.
