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Related Experiment Videos

Theta particles: a structure found in hamster sarcoma virus.

A P Albino, E DeHarven, F K Sanders

    Journal of Virology
    |September 1, 1972
    PubMed
    Summary

    Hamster sarcoma virus (HaSV) can form unusual "theta particles" when concentrated, unlike other leukemia viruses. This unique structure suggests differences in HaSV

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    Area of Science:

    • Virology
    • Electron Microscopy
    • Molecular Biology

    Background:

    • Hamster sarcoma virus (HaSV) is a type C ribonucleic acid tumor virus.
    • Standard electron microscopy of pelleted HaSV shows type C morphology.
    • Virus concentration methods can alter observed viral structures.

    Purpose of the Study:

    • To investigate the structural changes in HaSV after specific concentration and purification methods.
    • To characterize the atypical morphology observed in concentrated HaSV.
    • To compare HaSV morphology with other retroviruses under similar conditions.

    Main Methods:

    • Pelleting of HaSV from tissue culture fluid.
    • Concentration of virus using polyethylene glycol or ammonium sulfate.
    • Density gradient banding for purification.
    • Electron microscopy for morphological analysis.

    Main Results:

    • Concentrated HaSV exhibits an atypical barred core structure, termed "theta particles."
    • Theta particles suggest a condensed, flat disc-like ribonucleoprotein structure.
    • Atypical particles were exclusively observed in HaSV, not in similarly treated feline leukemia virus or Rauscher-murine leukemia virus.

    Conclusions:

    • The unique "theta particle" structure is specific to HaSV under tested conditions.
    • Differences in viral composition likely account for the formation of theta particles in HaSV.
    • Further research into HaSV composition is warranted to understand this phenomenon.

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