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Molecular characterization of curry leaf genotypes using ISSR markers
Dharini Chittaragi1, M Ananthan2, K Venkatesan3
1Department of Spices and Plantation Crops, Tamil Nadu Agricultural University, Coimbatore, 641003, India. chitti.smile3@gmail.com.
Scientific Reports
|October 30, 2025
Summary
Genetic diversity in curry leaf (Murraya koenigii) was assessed using Inter simple sequence repeat (ISSR) markers. This study reveals significant variability, crucial for improving curry leaf cultivation and germplasm management in South India.
Area of Science:
- * Agricultural Science
- * Plant Genetics
- * Molecular Biology
Background:
- * Murraya koenigii (curry leaf) is a vital tree spice from the Rutaceae family.
- * Variability assessment is essential for crop improvement programs.
- * South Indian curry leaf genotypes require evaluation for genetic diversity.
Purpose of the Study:
- * To evaluate genetic variability among curry leaf genotypes in South India.
- * To profile curry leaf accessions using Inter simple sequence repeat (ISSR) markers.
- * To inform germplasm management and breeding strategies for curry leaf.
Main Methods:
- * Collection of thirty curry leaf accessions from various South Indian locations.
- * Molecular profiling using 13 Inter simple sequence repeat (ISSR) markers.
- * Analysis of genetic diversity using Polymorphic Information Content (PIC) values and dendrogram clustering.
Main Results:
- * High average PIC value (0.83) indicated efficient diversity revelation by ISSR markers.
- * UBC-856, UBC-807, UBC-834, and UBC-835 markers showed PIC values > 0.5.
- * Genotypes were grouped into nine clusters based on similarity index, with Cluster I being the largest (15 genotypes).
Conclusions:
- * Significant genetic variability was observed among curry leaf genotypes using ISSR markers.
- * Findings support efficient germplasm management and selection of superior genotypes for breeding.
- * The study provides a basis for curry leaf conservation and cultivation strategies in South India.
