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Updated: Aug 10, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
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Correction: Discovery of therapeutic AGC2 modulators by combining docking, binding, and vesicle-based transport

Lucas Cafferati Beltrame1, Sabino Todisco1, Anna Lucia Francavilla1

  • 1Department of Biosciences, Biotechnologies and Environment, University of Bari "Aldo Moro", Via E. Orabona 4, 70125, Bari, Italy.

Journal of Translational Medicine
|October 30, 2025
PubMed
Abstract

No abstract available in PubMed .

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...