Related Experiment Video For Human papillomavirus
Updated: Jan 12, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Genetic variability and phylogenetic analysis of the long control region in human papillomavirus types 18 and 51 from
Zicong Lin1, Ting Li1, Yang Yang1
1Department of Laboratory Medicine, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, 434020, China.
Background:
High-risk human papillomavirus (HR-HPV) is the main pathogenic factor of cervical cancer. Both HPV-18 and HPV-51 belong to HR-HPV.
Methods:
Clinical samples were collected and processed to isolate single-positive HPV-18 and HPV-51 specimens. These specimens were then subjected to amplification of long control region (LCR) fragments and sequencing. Finally, a set of bioinformatic analyses was performed, including the evaluation of sequence variations, construction of phylogenetic trees, and prediction of transcription factor binding sites.
Results:
A total of 43 HPV-18 LCRs were successfully sequenced, yielding 17 single nucleotide polymorphisms (SNPs), while sequencing of 56 HPV-51 LCRs revealed 42 SNPs. Phylogenetic analysis demonstrated that HPV-18 variants clustered exclusively within lineage A, whereas HPV-51 variants were predominantly grouped in the same lineage. JASPAR predictions indicated potential functional consequences, with 11 HPV-18 mutation sites and 12 HPV-51 mutation sites possibly disrupting transcription factor binding sites.
Conclusion:
This study describes the genetic variations of the LCR of HPV-18 and HPV-51 in central China, which may disrupt binding sites of transcription factors. It provides important insights into the evolutionary characteristics and potential carcinogenic mechanisms of HPV in Central China.
