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Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Dendritic NIR-II Cyanines Enable Deep Imaging and Precise Surgery.

Bo He1, Zhehao Wang1, Yuji Sun1

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)
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Summary
This summary is machine-generated.

New poly(L-lysine) dendrimers offer enhanced stability and brightness for near-infrared II (NIR-II) imaging. These novel fluorophores improve tumor contrast and enable precise surgical guidance, outperforming existing agents.

Keywords:
NIR‐II fluorescence imagingcyanine dendrimersimage‐guided surgerymolecular engineeringtumor‐targeted imaging

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Area of Science:

  • Biomaterials Science
  • Medical Imaging
  • Organic Chemistry

Background:

  • High-performance near-infrared II (NIR-II) fluorophores are crucial for deep-tissue imaging but face challenges like poor stability and low quantum yield.
  • Existing NIR-II agents often exhibit limited tumor contrast and aqueous stability, hindering clinical translation.

Purpose of the Study:

  • To engineer generation-tunable poly(L-lysine) dendrimers (Cy-NIR-II-Gx) with a covalently integrated hydrophilic cyanine core for improved NIR-II fluorescence imaging.
  • To address fundamental challenges in NIR-II fluorophore design, including aqueous stability, quantum yield, and tumor contrast.

Main Methods:

  • Covalent integration of a hydrophilic cyanine core (Cy-NIR-II-NH2) into poly(L-lysine) dendrimers.
  • Utilizing dendritic architecture to suppress H-aggregation and prevent aqueous quenching.
  • Evaluating photostability, quantum yield in water, and deep-tissue imaging capabilities.

Main Results:

  • Achieved a 24.3-fold quantum yield enhancement (up to 0.802% in water) and >83% photostability retention after 48-h irradiation.
  • Demonstrated high-contrast vascular/lymphatic imaging with deep-tissue penetration and superior stability compared to indocyanine green (ICG).
  • Cy-NIR-II-G8 showed specific tumor accumulation in 4T1 breast tumors, maintaining a tumor-to-normal ratio >5 for 7 days.

Conclusions:

  • The engineered dendrimers effectively overcome key limitations of current NIR-II fluorophores, offering enhanced performance and stability.
  • Demonstrated significant potential for clinical applications, including precise tumor resection guidance and intraoperative margin delineation.
  • This molecular engineering strategy provides a robust platform for developing next-generation NIR-II imaging agents.