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Related Experiment Video

Updated: Jan 12, 2026

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

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Human three-dimensional engineered muscle tissue characterization and intramyocellular lipid modeling.

Tianxin Cao1, Eric Finnemore1,2, Jon Hill3

  • 1Cardiovascular-Renal Metabolic Diseases Research Department, Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, CT, USA.

Journal of Tissue Engineering
|October 30, 2025
PubMed
Summary

Engineered muscle tissues (EMTs) from primary human myoblasts show superior force generation and mature structure compared to those from induced pluripotent stem cell-derived myoblasts. This highlights their potential for disease modeling.

Keywords:
3D tissue engineeringcontractilityintramyocellular lipidskeletal muscle

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Area of Science:

  • Biomedical Engineering
  • Skeletal Muscle Physiology
  • Regenerative Medicine

Background:

  • Three-dimensional engineered muscle tissues (EMTs) are vital for in vitro studies of skeletal muscle.
  • Comparing primary human myoblasts (hP-Myo) and induced pluripotent stem cell-derived myoblasts (hiPS-Myo) is crucial for optimizing tissue models.

Purpose of the Study:

  • To comprehensively compare hP-Myo-derived EMTs and hiPS-Myo-derived EMTs.
  • To evaluate structural, functional, and transcriptional differences.
  • To assess the utility of hiPS-Myo EMTs in modeling metabolic dysfunction.

Main Methods:

  • Engineered muscle tissues (EMTs) from hP-Myo and hiPS-Myo.
  • Magnetic force-sensing platform for contractile performance analysis.
  • Histological assessment of tissue architecture.
  • Transcriptomic profiling.
  • Lipid overload model for metabolic dysfunction.

Main Results:

  • hP-Myo EMTs generated ~2-fold higher twitch forces and enhanced tetanic responses.
  • hP-Myo EMTs exhibited larger myofiber diameters and a mature skeletal muscle transcriptional signature.
  • hiPS-Myo EMTs subjected to lipid overload showed intracellular lipid accumulation, impaired contractility, and altered lipid metabolism.

Conclusions:

  • hP-Myo EMTs offer superior structural and functional maturity for skeletal muscle modeling.
  • hiPS-Myo EMTs can recapitulate key aspects of muscle metabolic dysfunction, such as lipid accumulation.