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Published on: September 16, 2022
Synthesis, Structural Analysis, and Antifungal Potential of Copper Oxide Nanoparticles against Candida Species
Arti Zende1, Jayant Pawar1, Chitra Khanwelkar2
1Department of Biotechnology, Krishna Institute of Science and Technology, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, Maharashtra, India.
Introduction:
Recurrent vaginal infections caused by Candida species, particularly drug-resistant strains, remain a major concern in women's reproductive health.
Aim:
This study evaluates the antifungal potential of copper oxide nanoparticles (CuO NPs), synthesized via a semi-solvothermal method, against clinically relevant vaginal Candida species-Candida albicans, C. glabrata, C. tropicalis, and C. krusei.
Materials And Methods:
Structural and optical characterization confirmed a crystalline cubic phase (XRD), a strong UV-Vi's absorption at 340 nm with a 3.4 eV band gap, and spherical nanoparticles (10-25 nm) seen via FESEM. Antifungal activity was tested using agar well diffusion and CFU assays across 15-1000 μg/mL concentrations.
Result:
A concentration-dependent colony forming unit (CFU) reduction was observed, with C. krusei showing the highest sensitivity (MIC: 60 μg/mL; complete inhibition at 1000 μg/mL), followed by C. glabrata. C. albicans and C. tropicalis exhibited moderate to high resistance.
Conclusion:
These findings highlight the potential of CuO NPs as a promising antifungal agent, particularly effective against resistant or non-albicans Candida species, offering an alternative strategy for treating vaginal candidiasis.
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