Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adjunctive Mechanical Ventilation or Intravenous Hypertonic Saline in Malawian Children with Cerebral Malaria and Highly Increased Brain Volume: A Randomized, Phase 3 Clinical Trial.

Neurocritical care·2026
Same author

Free Hemoglobin Increases Endothelial Adhesiveness to Red Blood Cells via a Toll-like Receptor 4-Dependent Mechanism.

The Journal of surgical research·2026
Same author

Executive Summary: Surviving Sepsis Campaign International Guidelines for the Management of Sepsis and Septic Shock in Children 2026.

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·2026
Same author

Surviving Sepsis Campaign International Guidelines for the Management of Sepsis and Septic Shock in Children 2026.

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·2026
Same author

Surviving Sepsis Campaign International Guidelines for the Management of Sepsis and Septic Shock in Children 2026.

Intensive care medicine·2026
Same author

Macrophages on demand: How tissue trauma shapes their role.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026

Related Experiment Video

Updated: Jan 12, 2026

A Data-Driven Approach to Quantifying Immune States in Sepsis
07:42

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

471

Quantifying Nuance Within Sepsis-Associated Immune Suppression Toward Diagnostic Certainty.

Charles C Caldwell1, Robert Maile2, Monty B Mazer3

  • 1Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio.

Shock (Augusta, Ga.)
|October 30, 2025
PubMed
Summary

Sepsis survivors maintain intact interferon-gamma (IFNγ) production after T-cell stimulation. However, sepsis non-survivors show heightened IFNγ responses to stimuli not involving the T-cell receptor (TCR).

More Related Videos

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
07:30

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

Published on: June 15, 2019

10.5K
Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
04:32

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

Published on: June 28, 2018

12.4K

Related Experiment Videos

Last Updated: Jan 12, 2026

A Data-Driven Approach to Quantifying Immune States in Sepsis
07:42

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

471
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
07:30

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

Published on: June 15, 2019

10.5K
Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
04:32

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

Published on: June 28, 2018

12.4K

Area of Science:

  • Immunology
  • Critical Care Medicine
  • Cellular Biology

Background:

  • Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection.
  • T-cell dysfunction is a known complication of sepsis, but its precise impact on immune responses and patient outcomes requires further elucidation.
  • Interferon-gamma (IFNγ) is a critical cytokine involved in T-cell mediated immunity.

Purpose of the Study:

  • To investigate the functional status of human T-cells during sepsis.
  • To correlate T-cell function with patient outcomes (survival vs. non-survival).
  • To assess the specific impact of sepsis on IFNγ production by T-cells.

Main Methods:

  • Human subjects with sepsis were studied.
  • T-cell function was assessed through in vitro stimulation assays.
  • Interferon-gamma (IFNγ) production was measured in response to various stimuli, including T-cell receptor (TCR)-dependent and TCR-independent pathways.
  • Comparison was made between sepsis survivors and non-survivors.

Main Results:

  • Sepsis survivors exhibited preserved IFNγ production capacity following T-cell stimulation.
  • Sepsis non-survivors demonstrated an exaggerated IFNγ response when T-cells were stimulated via TCR-independent pathways.
  • These findings suggest a differential impact of sepsis on distinct T-cell activation pathways.

Conclusions:

  • T-cell function, specifically IFNγ production, is altered in sepsis patients.
  • Preserved IFNγ response to TCR-stimulation in survivors indicates retained adaptive immunity.
  • Exaggerated IFNγ response to TCR-independent stimuli in non-survivors may contribute to detrimental inflammation and poor outcomes.