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Updated: Jan 12, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Comparative study on Acetobacter-mediated calcium release and speciation from diverse animal-derived calcium powders:
Jiani Lu1, Zhongqiang Wang1, Jinxuan Cao1
1Key Laboratory of Geriatric Nutrition and Health, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.
Abstract:
This study developed a bio-enhanced acidolysis process using Acetobacter fermentation to efficiently release and transform calcium from animal-derived byproducts, including eggshell, oyster shell, beef bone, and chicken bone powders. Shell-derived broths released markedly more calcium than bone-derived broths, around a 5.6-fold difference. Released calcium may be present in multiple forms: free ionic Ca2+, protein calcium, peptide calcium, and chelated complexes with organic and amino acids. FTIR, XRD, and BET analyses indicated that biologically produced acetic acid selectively dissolved disordered or amorphous regions, increasing residual crystallinity and particle size. Furthermore, the microbial system integrates organic acid generation with acid-driven mineral dissolution and collagen matrix loosening, forming a synergistic mechanism for calcium solubilization. Overall, this Acetobacter mediated bio-acidification process provides a green, economical, and multifunctional pathway for converting low-value animal byproducts into bioaccessible calcium ingredients, supporting sustainable development in functional food bioprocessing.
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