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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

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Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
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Microtubule Instability02:17

Microtubule Instability

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Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
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Centrosome Duplication02:25

Centrosome Duplication

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The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
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Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

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During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
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Spindle Assembly02:50

Spindle Assembly

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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
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Destabilization of Microtubules01:45

Destabilization of Microtubules

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The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
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Related Experiment Video

Updated: Jan 12, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

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Mechanisms underlying centriole stability.

Erica Biven1, Jennifer T Wang1

  • 1Department of Biology, Washington University in St Louis, St. Louis, Missouri, USA.

The Journal of Biological Chemistry
|October 30, 2025
PubMed
Summary
This summary is machine-generated.

Centrioles and basal bodies are stable microtubule structures essential for cell organization. Their stability relies on specific substructures, and regulated loss of these can lead to centriole elimination.

Keywords:
A-C linkerbasal bodiescartwheelcentriole eliminationcentriole losscentriolescentrosomesciliainner scaffoldmicrotubulespericentriolar materialprogrammed centriole lossprotein turnover

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Area of Science:

  • Cell Biology
  • Structural Biology

Background:

  • Centrioles and basal bodies are stable microtubule structures crucial for cell function.
  • They form the centrosome, the primary microtubule organizing center in animal cells.
  • Basal bodies template cilia formation, vital for signaling and motility.

Purpose of the Study:

  • To review recent advances in understanding centriole and basal body stability.
  • To explore the role of specific substructures in maintaining stability.
  • To discuss how regulated loss of substructures triggers centriole elimination.

Main Methods:

  • Review of recent scientific literature.
  • Analysis of structural components of centrioles and basal bodies.
  • Discussion of mechanisms of centriole elimination.

Main Results:

  • Centrosomal substructures like microtubule walls, cartwheel, and inner scaffold contribute to stability.
  • Pericentriolar material also plays a role in the long-term stability of these structures.
  • Regulated loss of these substructures is linked to centriole elimination.

Conclusions:

  • Specific substructures are key to the remarkable stability of centrioles and basal bodies.
  • Understanding these structures provides insight into regulated centriole elimination processes.
  • This knowledge is relevant for cell signaling, motility, and developmental biology.