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Updated: Aug 14, 2026
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Dual-mode fluorescence/colorimetric aptasensor based on Zn,Ce-MOF nanocomposite for sensitive detection of
Yimeng Li1, Jiayao Wang2, Yi Ran3
1Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs (MARA) / Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environment Protection Institution, MARA, Tianjin, 300191, PR China; College of Life Science, Yangtze University, Jingzhou, 434023, Hubei Province, PR China.
Background:
A dual-mode fluorescence/colorimetric aptasensor was developed for the sensitive detection of isocarbophos, an organophosphorus pesticide posing significant public health risks. Using Zn,Ce-MOF nanocomposites, this sensor integrated magnetic separation and signal amplification. This integrated biosensing strategy offers a promising tool for environmental safety assurance and food quality control applications.
Results:
The Zn,Ce-MOF nanozyme of this sensor serves as both a carrier of 3,3',5,5'-tetramethylbenzidine (TMB) and oxidase mimic, facilitating enzyme-free TMB oxidation. The dual-readout system enables self-validating detection through independent channels (fluorescence LOD: 0.06 ng mL-1; colorimetric LOD: 0.09 ng mL-1), with robust performance, high interferent selectivity, and reproducibility. Practical validation in spiked agricultural samples yielded recovery rates of 97.7-113.6 %, confirming the platform's suitability for field-deployable pesticide monitoring.
Significance:
This work developed a fluorescence/visual aptasensor with self-assembled TMB/Au/Zn,Ce-MOF probes for one-step magnetic separation dual-mode detection. This integrated biosensing strategy improves detection efficiency and accuracy, offering a general strategy for multimodal biosensors.
