Related Experiment Video
Updated: Jan 12, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Complete genome sequencing of 18 urea-utilizing Vibrio strains isolated from coral
Kefan Li1,2, Wei Lin1,2, Jiebin Zhou1,2
1Guangdong Provincial Key Laboratory of Marine Biology and Department of Biology, Shantou University, Shantou, 515063, China.
Abstract:
Vibrio species are recognized as potential causative agents of coral diseases, yet their metabolic capabilities concerning different nitrogen sources, such as urea, have been largely understudied. This study focuses on 18 urea-utilizing Vibrio strains isolated from coral, for which we performed complete genome sequencing using Illumina technology. The average assembled genome size was 5.67 Mb, with an average contig N50 of 0.61 Mb and scaffold N50 of 0.61 Mb. We predicted an average of 5,087 protein-coding genes per genome, with over 95% of these genes successfully annotated for function. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis revealed an average 98.9% completeness for the Vibrio spp. lineage. Overall, this study provides high-quality genomic data for these Vibrio strains, offering a valuable resource for further research into their evolution, molecular mechanisms of pathogenicity, and their specific roles in the nitrogen cycle of the coral ecosystem.
More Related Videos
10:43Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Published on: November 5, 2014
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Other Unique Bacteria
Bacterial Phylum Verrucomicrobiota
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...