Regulating white blood cell activity through the novel universal receptive system

Area of Science:

• Immunology and Cell Biology
• Molecular Biology
• Biotechnology

Background:

• Understanding immune cell regulation in disease is limited.
• Novel strategies for immune cell manipulation are needed.
• Nucleic acid-based receptors (TezRs) influence cell responses.

Purpose of the Study:

• Investigate the Universal Receptive System for immune cell regulation.
• Target nucleic acid receptors on leukocytes to generate modified cells.
• Assess the functional impact of these modified immune cells.

Main Methods:

• Targeted leukocytes using endonucleases to modify surface-bound DNA- and RNA-based receptors.
• Generated modified cells termed "Leukocyte-Tells."
• Assessed gene expression, antimicrobial, anticancer, and cytokine production.

Main Results:

• Upregulation of 1,496 genes, including immune cell signaling, migration, endocytosis, and phagocytosis pathways.
• Leukocyte-Tells demonstrated significantly enhanced antimicrobial and anticancer activities in vitro.
• Increased production of key cytokines like TNF, IL-1β, IFN-γ, IL-6, and IL-10.

Conclusions:

• The Universal Receptive System can orchestrate fundamental immune cell properties.
• Leukocyte-Tells exhibit superior antimicrobial and anti-tumor functions.
• This approach provides new insights into leukocyte biology and cell-based therapies.