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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Stage-specific TRIM10 expression regulates erythroid maturation.

Heesoo Kim1, Wonji Shin2, Dongeun Lee1

  • 1Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, South Korea.

EMBO Reports
|October 31, 2025
PubMed
Summary
This summary is machine-generated.

Tripartite motif-containing protein 10α (TRIM10α) is vital for red blood cell maturation, aiding hemoglobin processing and clearance. Its selective expression prevents harmful aggregation, supporting erythroid homeostasis and disease insights.

Keywords:
C1qErythropoiesisHemoglobin MaturationTRIM10

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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Area of Science:

  • Cell Biology
  • Hematology
  • Molecular Biology

Background:

  • Erythroid cells undergo significant remodeling during differentiation.
  • Ubiquitin E3 ligases are dynamically regulated during erythropoiesis, but their functions beyond catalysis are not fully understood.

Purpose of the Study:

  • To investigate the role of tripartite motif-containing protein 10α (TRIM10α) in erythroid maturation.
  • To elucidate the mechanisms by which TRIM10α regulates red blood cell development and homeostasis.

Main Methods:

  • Identification of TRIM10α as an erythroid-specific E3 ligase.
  • Analysis of TRIM10α self-association, localization, and interaction with complement C1q.
  • Investigation of TRIM10α's role in hemoglobin maturation, aggregation, and degradation pathways (lysosomal and autophagic).
  • Comparative analysis with the alternatively spliced TRIM10β isoform.

Main Results:

  • TRIM10α self-associates to bind extracellular C1q on erythroblast surfaces, facilitating macrophage recognition and pyrenocyte encapsulation.
  • Surface C1q interacts with EpoR, promoting lysosomal degradation and modulating erythropoietin signaling.
  • Cytosolic TRIM10α enhances hemoglobin maturation and sequesters aggregates under oxidative stress.
  • TRIM10α degradation via p62-mediated autophagy clears hemoglobin aggregates.
  • The alternatively spliced TRIM10β isoform, poorly expressed in humans, forms deleterious aggregates, suggesting its evolutionary suppression is crucial for erythroid homeostasis.

Conclusions:

  • TRIM10α plays a multifaceted role in stepwise erythroid maturation, regulating cell surface interactions, hemoglobin processing, and aggregate clearance.
  • Aberrant TRIM10 expression is implicated in erythroid-related diseases.
  • TRIM10 represents a potential biomarker and therapeutic target for hematological disorders.