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Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

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Published on: March 24, 2017

ZNF334 truncation mutation drives cold-induced autoinflammation.

Joung-Liang Lan^1,2,3, Shih-Hsin Chang^3,4, Yi-Hua Lai^1,2,3

¹College of Medicine, China Medical University, Taichung, Taiwan.

EMBO Molecular Medicine

|October 31, 2025

Summary

Zinc finger protein 334 (ZNF334) regulates cold-induced inflammation. A ZNF334 mutation impairs heat shock protein 90 (Hsp90) interaction, causing autoinflammatory disease. Hsp90 inhibitors may treat cold-induced inflammatory conditions.

Keywords:

Autoinflammation Cold Mutation Sensorineural Hearing Loss ZNF334

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Area of Science:

Immunology
Genetics
Molecular Biology

Background:

The function of zinc finger protein 334 (ZNF334) in immunity is unclear.
Autoinflammatory diseases can be triggered by environmental factors like cold exposure.

Purpose of the Study:

To investigate the role of ZNF334 in cold-induced inflammation and autoinflammatory disease.
To elucidate the molecular mechanisms underlying ZNF334 deficiency-related pathology.

Main Methods:

Identified a ZNF334 truncation mutation in a patient with cold-induced autoinflammatory disease.
Utilized patient-derived monocytes and CRISPR/Cas9-edited THP-1 cells.
Analyzed protein interactions, cellular stress responses, and inflammatory signaling pathways.

Main Results:

The ZNF334 mutation disrupted ZNF334-Hsp90 interaction, reducing cold stress regulators (Hsp90, TRPM8).
Mutation led to ER stress, impaired redox homeostasis, and increased cold-induced NF-κB activation.
Monocytes from the patient exhibited enhanced secretion of pro-inflammatory extracellular vesicles containing mitochondria.
Cold avoidance improved patient symptoms; Hsp90 inhibition in cells reduced cold-induced inflammation.

Conclusions:

ZNF334 is crucial for regulating cold-induced inflammation and oxidative stress.
Hsp90 ATPase inhibitors show potential for treating autoinflammatory diseases triggered by cold exposure.