[Establishing the dynamics of activity and stability of total PSA samples in semen samples on gauze, diluted with...

The article presents the results of an experimental study of the stability of total PSA in samples from sperm dried on gauze, diluted with distilled water. The goal of the work was to establish the dynamics of activity and stability of total PSA samples in the prepared extracts. The research material included samples of seminal fluid dried on gauze. The study was carried out using a quantitative enzyme-linked immunosorbent assay. Measurements in the samples were carried out 5 times during the working day. The results obtained allowed us to prove a significant decrease in total PSA activity in extracts from sperm stains on gauze for 6 hours at room temperature (+18-20 °C). The results of the study, confirmed by statistical analysis data, can be taken into account in the case of using the quantitative enzyme immunoassay method to determine the presence of sperm on material evidence during forensic biological examinations.