Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Emerging Technologies in RNA-Protein Interaction Analysis.

Biology·2026

Same author

Anti-Epstein-Barr virus (EBV) antibodies in EBV-associated gastric carcinoma.

Infectious agents and cancer·2026

Same author

Large-Scale Saliva-Based Clinical Surveillance Enables Real Time SARS-CoV-2 Outbreak Detection and Genomic Tracking (Arizona, 2020-2023).

Diagnostics (Basel, Switzerland)·2025

Same author

Nucleic Acid Programmable Protein Arrays for Autoantibody Discovery: A Step-by-Step Guide.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Progressive multifocal leukoencephalopathy and BK virus-nephropathy with bispecific antibody therapy in multiple myeloma.

Haematologica·2025

Same author

RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks.

Scientific reports·2025

Same journal

Scalable Synthesis of Calcium Fluoride Nanoparticles as a Novel Ultrasound Contrast Agent for Imaging Tumor Targeted Delivery of Therapeutics.

Chemical & biomedical imaging·2026

Same journal

Bipolar Electrochemiluminescence at Single Gold Microbeads Trapped by Micropipettes.

Chemical & biomedical imaging·2026

Same journal

Multiplexed Detection of Reactive Biomolecules via Chemoresponsive DNA Accumulation on Fluorescence-Encoded Beads.

Chemical & biomedical imaging·2026

Same journal

Peptide-Based pH-Responsive MRI-CEST Agents: In Vivo Comparison between a Selected Pentapeptide and the Established Iopamidol Reference in Tumor pH Mapping Ability.

Chemical & biomedical imaging·2026

Same journal

Red Blood Cell-Encapsulated Nanoparticles for Long-Circulating, Improved Specificity Functional MRI.

Chemical & biomedical imaging·2026

Same journal

Bioimaging of Metals.

Chemical & biomedical imaging·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 12, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Highly Sensitive Spatial Proteomics with Multicolor Cleavable Fluorescent Tyramide.

Yi Chen¹, Yu-Sheng Wang¹, Joshua Labaer¹

¹Biodesign Institute & School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States.

Chemical & Biomedical Imaging

|October 31, 2025

Summary

This summary is machine-generated.

This study introduces a sensitive spatial proteomics method for high-resolution cell analysis. The technique enables multiplexed protein profiling in tissues, revealing cell clusters and interactions.

Keywords:

Immunofluorescence Immunohistochemistry imaging multiplex single-cell

More Related Videos

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Related Experiment Videos

Last Updated: Jan 12, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Area of Science:

Biochemistry
Cell Biology
Proteomics

Background:

High-resolution single-cell spatial proteomics provides critical insights into biological systems.
Current multiplexed protein imaging platforms have limitations in sensitivity, multiplexing capacity, and technical complexity.

Purpose of the Study:

To develop a highly sensitive spatial proteomics approach for multiplexed protein profiling.
To overcome the limitations of existing protein imaging platforms.

Main Methods:

Utilized multicolor cleavable fluorescent tyramide and off-the-shelf antibodies.
Employed horseradish peroxidase (HRP) for enzymatic fluorophore deposition and reiterative cycles of labeling, imaging, and cleavage.
Applied the method to human formalin-fixed paraffin-embedded (FFPE) tonsil tissue.

Main Results:

Quantified 38 proteins in FFPE tonsil tissue, achieving the highest multiplexing capacity with tyramide signal amplification (TSA) methods to date.
Analyzed approximately 500,000 individual cells, identifying distinct cell clusters based on protein expression and spatial microenvironment.
Observed specific tissue subregions composed of unique cell clusters and characterized cell-cell interactions, with cells in the same cluster showing association and cells in different clusters avoiding contact.

Conclusions:

The developed spatial proteomics approach enables high-resolution, multiplexed protein profiling in FFPE tissues.
The method facilitates the discovery of tissue-specific cell clusters and their spatial organization.
Provides novel insights into cell-cell interactions within complex biological microenvironments.