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Related Experiment Video

Updated: Jan 12, 2026

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography
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Device-based day-to-day and observer variability to quantify dilation capacity in the retinal microcirculation.

Lukas Streese1,2, Christoph Hauser1, Denis Infanger1

  • 1Department of Sport, Exercise and Health, Medical Faculty, University of Basel, Basel, Switzerland.

Frontiers in Physiology
|October 31, 2025
PubMed
Summary

Dynamic retinal analysis (DVA) shows moderate day-to-day variability but excellent intra- and interobserver reliability. Adjusting for baseline diameter may improve DVA

Keywords:
clinical implementationdevicedynamic retinal vessel analysisreproducibilityretinal endothelial function

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Area of Science:

  • Ophthalmology and visual sciences
  • Medical technology and devices
  • Cardiovascular research

Background:

  • Dynamic retinal analysis (DVA) is a validated method for quantifying microvascular endothelial function.
  • Assessing the reliability of DVA is crucial for its clinical implementation.
  • Understanding variability is key to standardizing DVA protocols.

Purpose of the Study:

  • To evaluate day-to-day, intra- and interobserver variability of DVA.
  • To compare DVA measurements between two device generations (DVA 2.0 and DVA 3.0).
  • To identify factors influencing DVA reliability.

Main Methods:

  • DVA measurements were taken on two separate days using DVA 2.0 and DVA 3.0 devices.
  • Intraobserver variability was assessed by one reader analyzing 20 arteriolar (aFID) and venular flicker-light induced dilation (vFID) signals twice.
  • Interobserver variability was assessed by a second reader independently analyzing 20 aFID and vFID signals. Interclass correlation coefficient (ICC) was used.

Main Results:

  • Moderate to good day-to-day variability was observed for both aFID and vFID across both DVA versions.
  • Excellent intraobserver (aFID/vFID: 0.999) and interobserver (aFID: 0.997, vFID: 0.998) reliability was demonstrated.
  • Moderate interdevice variability was found between DVA 2.0 and DVA 3.0 (aFID: 0.76, vFID: 0.87). ICC improved after correcting for baseline diameter.

Conclusions:

  • DVA exhibits reliable intra- and interobserver performance.
  • Day-to-day and interdevice variability exist, suggesting a need for standardization.
  • Accounting for arteriolar baseline diameter variations can enhance DVA reliability in research and clinical settings.