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The Prevalence Rates of Colistin Resistance Among Third-Generation Cephalosporin-Resistant E. coli Isolates From Thai

Siriporn Kowaboot1, Nipaporn Tewawong1, Apichai Sreepian1

  • 1Faculty of Medical Technology, Rangsit University, Pathum Thani, Thailand.

Thescientificworldjournal
|October 31, 2025
PubMed

Insights

Colistin resistance in E. coli is rising. A study in Thailand found 1.45% of E. coli isolates resistant to colistin, with some carrying mcr genes, highlighting the need for better antibiotic control.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Rising antibiotic resistance, including colistin resistance, is a global health threat.
  • Colistin resistance is emerging in Enterobacterales, often alongside resistance to other critical antibiotics like cephalosporins and carbapenemens.
  • The inappropriate use and overuse of antibiotics contribute significantly to the development of drug-resistant infections.

Purpose of the Study:

  • To determine the prevalence of colistin resistance in third-generation cephalosporin-resistant Escherichia coli (E. coli) isolates from patients in Thailand.
  • To identify the presence of specific colistin resistance genes (mcr genes) in these resistant E. coli isolates.
  • To provide data for antibiotic resistance surveillance and inform public health strategies.

Main Methods:

  • Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of colistin.
  • Multiplex polymerase chain reaction (PCR) was employed to detect mcr-1 and mcr-3 genes.
  • Analysis of 343 E. coli isolates obtained from patients at Nopparat Rajathanee Hospital in 2023.

Main Results:

  • A prevalence of 1.45% (5/343) colistin-resistant E. coli was observed, with MIC values between 4 and 8 μg/mL.
  • Among the five colistin-resistant isolates, resistance genes were detected: two harbored mcr-1, two harbored mcr-3, and one carried both mcr-1 and mcr-3.
  • These findings indicate the presence of mobile colistin resistance genes in E. coli exhibiting resistance to other critical antibiotic classes.

Conclusions:

  • The study identified a low but significant prevalence of colistin-resistant E. coli in Thailand, carrying concerning mcr genes.
  • The presence of mcr-1 and mcr-3 genes underscores the potential for rapid dissemination of colistin resistance.
  • These findings emphasize the critical need for enhanced surveillance, antimicrobial stewardship, and public health interventions to combat the spread of antibiotic-resistant bacteria.
Keywords:
E. colicolistin resistancemcr-1mcr-3

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