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Updated: Jan 12, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Strategies for multimodal spatiotemporal profiling of phosphorylation in cilia biology
Rachel E Turn1, Mohammad Ovais Aziz-Zanjani1, Anushweta Asthana1
1Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Abstract:
This Opinion piece highlights recent advances in technical approaches to dissect the mechanisms coupling cell cycle and ciliogenesis during G0/quiescence and the importance of transient post-translational modifications (PTMs) as dynamic regulators of these processes. We discuss the latest technologies enabling real-time monitoring of context-dependent phosphoproteomics and emerging concepts in PTM-driven control of ciliary function. Additionally, we outline major unanswered questions and propose future research directions. A better understanding of G0 regulatory pathways might both spur the development of clinical interventions for human cilia-linked pathologies and set the stage for future research linking cell quiescence to cancer and tissue regeneration.
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