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An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

SERS-based mercury ion "on-off" dual-mode sensor using a silver cavity array substrate.

Xuefang Gu¹, Xiaofeng Ning¹, Jin Fu¹

¹School of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, Jiangsu, PR China.

|October 31, 2025

Summary

This summary is machine-generated.

A novel SERS sensor offers dual turn-on/turn-off modes for rapid, sensitive mercury ion detection. This advanced sensor utilizes a silver cavity array for precise environmental and food safety monitoring.

Keywords:

Glutathione mercury chelates Mercury ions Silver cavity array Silver mercury amalgam Surface-enhanced Raman scattering

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Area of Science:

Analytical Chemistry
Materials Science
Environmental Science

Background:

Mercury ions (Hg²⁺) pose significant environmental and health risks.
Existing methods for mercury detection often lack sensitivity, speed, or require complex sample preparation.
Development of rapid, highly sensitive, and selective sensors is crucial for effective monitoring.

Purpose of the Study:

To develop a novel Surface-Enhanced Raman Spectroscopy (SERS)-based sensor for mercury ion detection.
To achieve rapid, highly sensitive, and precise detection using a dual turn-on/turn-off mode.
To validate the sensor's performance in real-world samples like cultured clam and river water.

Main Methods:

Fabrication of a two-dimensional, highly ordered silver cavity array (SCA) substrate.
Utilizing the alloying reaction between mercury and SCA for turn-off mode detection.
Employing glutathione (GSH) and sodium thiophenolate (TP) for selective binding and signal amplification in the turn-on mode.
SERS signal analysis for quantitative detection of Hg²⁺.

Main Results:

The sensor demonstrated a dual turn-on/turn-off detection mechanism for Hg²⁺.
Achieved low detection limits of 0.3 nM (turn-on) and 0.027 nM (turn-off).
Experimental validation confirmed the sensor's rapidity, accuracy, and high sensitivity in spiked recovery assays of environmental samples.

Conclusions:

The developed SERS sensor provides a highly sensitive and rapid method for Hg²⁺ detection.
The dual mode approach offers enhanced analytical capabilities.
The sensor shows significant potential for environmental monitoring, food safety, and pollution early warning applications.