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Updated: Jan 12, 2026

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
Published on: June 27, 2022
Investigation of electron beam resistance of mAzamiGreen using an electron photon hybrid microscope
Mihiro Suzuki1, Toshiki Shimizu1, Hiroki Minoda1
1Department of Applied Physics and Chemical Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
Abstract:
Enhanced green fluorescent proteins (EGFP) emit cathodoluminescence upon irradiation with electron beams. This phenomenon can be leveraged as a promising tool for investigating biological processes. However, bleaching upon electron beam irradiation presents a challenge to its application. In this study, the electron beam resistance of mAzami Green (mAG), a fluorescent protein with higher structural stability than EGFP, was examined. Similar to EGFP, mAG exhibited a red shift and decreased fluorescence intensity upon electron beam irradiation. This decrease in fluorescence intensity was attributed to quenching by two different decomposition processes: direct quenching and redshift. Analysis of the effect of environmental conditions on the electron beam resistance revealed that the electron beam resistance was slightly higher in solution than in the dry state. This is likely because, in a liquid environment, material exchange with the environment facilitates structural repair, resulting in a decrease in the effective intensity decay rate.
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