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Isolation and characterization of a novel lytic bacteriophage vB_EcoS_P78 against multidrug-resistant Escherichia

Rong Wen1, Jiahao Tong1, Shoude Liu1

  • 1National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.

Virus Research
|October 31, 2025
PubMed

Insights

A novel bacteriophage, Escherichia coli phage vB_EcoS_P78, effectively lyses multidrug-resistant E. coli strains. This siphovirus shows broad host range and stability, indicating potential for phage therapy against resistant bacterial infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Multidrug-resistant (MDR) Escherichia coli poses a significant global health threat.
  • Bacteriophages offer a promising alternative or adjunct to conventional antibiotics.

Purpose of the Study:

  • To characterize a novel bacteriophage, vB_EcoS_P78, isolated from swine feces.
  • To evaluate its lytic activity against MDR E. coli clinical isolates.
  • To determine its genomic and phylogenetic characteristics.

Main Methods:

  • Isolation and characterization of bacteriophage vB_EcoS_P78.
  • Host range determination against ESBL-producing and MDR E. coli.
  • Stability assays (temperature, pH).
  • One-step growth curve analysis.
  • Whole-genome sequencing and phylogenetic analysis.

Main Results:

  • Phage vB_EcoS_P78 (siphovirus morphology) lysed 75% of tested MDR E. coli.
  • Demonstrated stability at temperatures up to 50°C and pH 3-10.
  • Exhibited a 20-minute latent period and burst size of 220 PFU.
  • Genome sequenced (44,574 bp dsDNA, 58 ORFs), lacking resistance/virulence genes.
  • Phylogenetic analysis placed it as a novel Dhillonvirus species.

Conclusions:

  • Phage vB_EcoS_P78 is a novel species within the Dhillonvirus genus.
  • Its broad host range, stability, and lytic efficacy suggest significant therapeutic potential against MDR E. coli infections.
Keywords:
BacteriophageDiarrheaEscherichia coliGenomic characteristicsMultidrug-resistant (MDR)Siphovirus

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