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Updated: Jan 12, 2026
Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
Published on: August 15, 2025
Isolation, structural characterization, molecular docking and hypoglycemic activities of an arabinose- and
Qian Zeng1, Zheyuan Liu1, Wenxing Ao1
1Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Abstract:
Rehmannia glutinosa, a widely used traditional Chinese medicinal herb and dietary therapeutic, is rich in polysaccharides. In this experiment, column chromatography was employed to separate and purify the polysaccharides from Rehmannia glutinosa, yielding the homogeneous polysaccharide RGP70-2-1. Structural analysis of RGP70-2-1 was conducted using various methods, including HPLC, FT-IR, GC-MS, NMR, and SEM. The results indicate that RGP70-2-1 is a high-molecular-weight structure composed of five monosaccharides (mannose, rhamnose, galactose, arabinose, and glucose), with 14 types of linkages. Building upon this structural framework, this study employed molecular docking and in vitro assays to demonstrate that RGP70-2-1 significantly inhibits α-glucosidase and α-amylase activities, indicating its potential hypoglycemic effect. The work enriches the primary structural information on Rehmannia glutinosa polysaccharides and lays a foundation for future investigations into their bioactivities and structure-function relationships.
