Protocol for analyzing shoot apical meristem development and heat stress responses in tomato

Shoot apical meristem (SAM) development is critical for plant aerial architecture and reproduction, but its spatiotemporal specificity and phenotyping challenges hinder mechanistic studies, particularly under stresses. Here, we present a protocol for molecular and cellular analysis of SAM development and stress response in tomato. We describe steps for imaging, developmental stage-specific heat treatment, and recovery growth. We then detail procedures for microdissection and tissue sectioning. This protocol enables high-resolution investigation of SAM development in response to heat stress. For complete details on the use and execution of this protocol, please refer to Huang et al.¹.