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Updated: Jan 12, 2026
Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
Published on: August 21, 2021
Succinylated chitosan/recombinant collagen III composite hydrogel with immunomodulatory and angiogenic properties for
Li Haojing1, Luo Xin2, Chen Zhiwan1
1Department of Material Science and Engineering, College of Chemistry and Materials, Jinan University, Guangzhou, 510632, PR China.
Abstract:
Chronic diabetic wounds remain challenging to treat due to persistent inflammation and inadequate vascularization. Here, we developed a multifunctional hydrogel by covalently integrating N-succinylated chitosan (NSC) with recombinant human collagen type III (rhCol III) via EDC/NHS cross-linking. The hydrogel exhibited robust mechanical strength, interconnected porosity, high swelling capacity, and stability. In vitro, it promoted endothelial cell proliferation, migration, and the secretion of nitric oxide and VEGF, while suppressing TNF-α and IL-1β and upregulating IL-10. In vivo, in a streptozotocin-induced diabetic mouse model, the hydrogel accelerated wound closure, enhanced re-epithelialization, increased collagen deposition, and stimulated neovascularization. These therapeutic effects arise from the synergistic immunomodulatory and pro-angiogenic functions of the NSC/rhCol III hydrogel. This multifunctional platform offers a promising strategy for promoting chronic diabetic wound repair.
