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Published on: September 5, 2013
Discovery of IS1515 transposition into the Spn9802 fragment of Streptococcus pneumoniae and its implications for
Myrthe M A Toorop1, Margriet E M Kraakman1, Eric C J Claas1
1Leiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, Leiden, The Netherlands.
Abstract:
This study investigates a diagnostic challenge in detecting Streptococcus pneumoniae using a Spn9802-targeted PCR assay. Four S. pneumoniae isolates tested lytA-positive but Spn9802-negative due to an insertion sequence (IS1515) within Spn9802, which was confirmed by whole-genome sequencing. This insertion may lead to false-negative results, posing concerns for PCR-based diagnostics. The study emphasizes the need for dual-target PCR and regular updates to diagnostic primers, underscoring the importance of continuous assay evaluation and post-market surveillance to ensure reliable pathogen detection.
