Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

33.4K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.4K
Overview of DNA Repair02:25

Overview of DNA Repair

9.6K
9.6K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.0K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.0K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.0K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.0K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

40.6K
Overview
40.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Essential Contributions of Ribose and Nucleobases to the Nucleophilic Reactivity of RNA 2'-OH Groups.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Correction to "DNA Content and DNA Damage in Raw and Heat-Processed Foods".

Journal of agricultural and food chemistry·2026
Same author

C‑Nucleosides Stabilize RNA by Reducing Nucleophilicity at 2'-OH.

ACS central science·2025
Same author

Fluorogenic Covalent Probes for RNA.

Journal of the American Chemical Society·2025
Same author

RNA 2'-OH modification with stable reagents enabled by nucleophilic catalysis.

RSC advances·2025
Same author

Sequence-Specific Installation of Aryl Groups in RNA via DNA-Catalyst Conjugates.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Jan 12, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K

DNA Content and DNA Damage in Raw and Heat-Processed Foods.

Jinwoo Shin1, Pawel Jaruga2, Miral Dizdaroglu2

  • 1Department of Chemistry, Stanford University, Stanford, California 94305, United States.

Journal of Agricultural and Food Chemistry
|November 3, 2025
PubMed
Summary

Cooking damaged food DNA, creating harmful nucleotides that human cells can absorb. This process may incorporate genotoxic species into host DNA, potentially increasing risks of cytotoxicity and DNA double-strand breaks.

Keywords:
DNA contentDNA damageDNA salvage, heat-processed foodsdeaminationgenotoxicity

More Related Videos

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

5.8K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.0K

Related Experiment Videos

Last Updated: Jan 12, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

5.8K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.0K

Area of Science:

  • Food Science
  • Molecular Biology
  • Toxicology

Background:

  • Dietary DNA provides nucleotides for cellular DNA synthesis.
  • Preliminary evidence suggests high-temperature cooking damages food DNA.
  • Damaged DNA fragments may be absorbed and incorporated into host DNA.

Purpose of the Study:

  • To assess the risk of consuming DNA from cooked foods.
  • To quantify DNA damage in various raw and roasted food ingredients.
  • To investigate the impact of damaged food DNA on human cell lines.

Main Methods:

  • Surveyed DNA content and damage in 21 food ingredients (raw and roasted).
  • Quantified oxidative (8-oxo-dG) and deaminated (dU) DNA damage.
  • Incubated human cell lines with damaged nucleosides from cooked foods.

Main Results:

  • Significant variation in extractable DNA content across food types.
  • Cooking caused up to 250-fold increases in oxidative and deaminated DNA damage.
  • Damaged nucleosides induced cytotoxicity and increased DNA double-strand breaks in cell lines.

Conclusions:

  • High-temperature cooking significantly elevates DNA damage in foods.
  • Consuming damaged food DNA may pose risks due to nucleotide salvage pathways.
  • Further research is needed to fully understand the toxicological implications for human health.