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Epigenetic Regulation01:37

Epigenetic Regulation

3.7K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.7K
Epigenetic Regulation01:46

Epigenetic Regulation

33.4K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.4K
Histone Modification02:32

Histone Modification

4.4K
4.4K
Histone Modification02:32

Histone Modification

15.9K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.9K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.1K
Genomics02:02

Genomics

39.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
39.6K

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Related Experiment Video

Updated: Jan 12, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

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Recent advances in methodologies of epigenomics.

Hiroaki Ohishi1, Wan Kin Au Yeung2

  • 1Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Epigenomics
|November 3, 2025
PubMed
Summary

Recent advances in epigenomics enable single-cell, multi-omic, and spatial mapping of the epigenome. These innovative technologies provide unprecedented resolution for understanding chromatin biology in health and disease.

Keywords:
Epigeneticsimagingintegrative approachlong-readmulti-omicssequencingsingle-cellspatial

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Last Updated: Jan 12, 2026

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Epigenomics has evolved from bulk assays to high-resolution single-cell investigations.
  • Technological advancements are key to this transformation.

Purpose of the Study:

  • To review interconnected technologies for mapping the epigenome.
  • To highlight biological insights and future directions in epigenomic research.

Main Methods:

  • Next-generation and long-read sequencing for chromatin accessibility, modifications, and 3D structure.
  • Live-cell probes and multiplexed chromatin tracing for dynamic organization visualization.
  • Integrative platforms merging sequencing data with 3D coordinates.

Main Results:

  • Mapping of chromatin accessibility, histone/DNA modifications, and 3D structure at single-cell resolution.
  • Visualization of dynamic epigenetic marks and genome architecture in intact cells/tissues.
  • Holistic view of gene regulation by integrating base-level reads with 3D coordinates.

Conclusions:

  • Innovations are redefining epigenomic research with unprecedented breadth and resolution.
  • Future directions include routine, cost-effective epigenomic investigations.
  • These technologies are crucial for interrogating chromatin biology in health and disease.