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Sendai virus-induced transcriptase from infected cells: polypeptides in the transcriptive complex.

H O Stone, D W Kingsbury, R W Darlington

    Journal of Virology
    |November 1, 1972
    PubMed
    Summary
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    Researchers isolated a Sendai virus transcriptase-template complex from infected cells. This complex, containing two key viral proteins, resembles viral nucleocapsids in structure and composition.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cellular Biology

    Background:

    • Sendai virus is an RNA virus known to replicate within the cytoplasm of infected host cells.
    • Viral transcriptase complexes are essential for RNA virus replication, synthesizing viral RNA from a template.

    Purpose of the Study:

    • To isolate and characterize the Sendai virus transcriptase-template complex.
    • To elucidate the protein composition and structural properties of this essential viral machinery.

    Main Methods:

    • Combined sedimentation and isopycnic centrifugation in D2O-sucrose gradients were used to isolate the complex from infected cell cytoplasm.
    • Buoyant density measurements, chemical composition analysis, and electron microscopy were employed for characterization.

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    Main Results:

    • A Sendai virus transcriptase-template complex was successfully isolated, banding at a buoyant density of 1.27 g/cm(3).
    • The complex contained two major polypeptides: the viral nucleocapsid structure unit (60,000 MW) and the largest virion polypeptide (75,000 MW).
    • Structural analysis indicated the complex closely resembles viral nucleocapsids.

    Conclusions:

    • The isolated complex represents a functional transcriptase-template entity involved in Sendai virus RNA synthesis.
    • The protein components and structural similarity to nucleocapsids provide insights into the mechanism of viral RNA replication.