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Updated: Jan 12, 2026
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
Protective effects of 7-hydroxyflavone on mitochondrial membrane potential and hydrogen peroxide-induced apoptosis in
Hsuan-Li Huang1,2, Lu-Kai Wang3, Fu-Ming Tsai4
1Division of Cardiology, Department of Internal Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei, Taiwan.
Objectives:
The objective is to evaluate the protective effects of six coumarin derivatives against peroxide-induced cardiomyocyte damage and investigate their action mechanisms.
Materials And Methods:
Intracellular reactive oxygen species and mitochondrial membrane potential (MMP) were analyzed using dihydrorhodamine 123 and JC-1 combined with flow cytometry. Cell viability and apoptosis were assessed using WST-1 and lactate dehydrogenase analysis kits, respectively. The apoptotic signaling pathway was analyzed using a mouse apoptosis array kit. Cellular protein expression was detected using Western blotting.
Results:
Among the six coumarin derivatives tested, only 7-hydroxyflavone demonstrated the ability to protect cardiomyocytes from hydrogen peroxide (H2O2)-induced damage. Protein expression analysis revealed that 7-hydroxyflavone reduced cytochrome c release from the mitochondria and inhibited H2O2-induced activation of caspase-3. In addition, 7-hydroxyflavone maintained MMP stability in cardiomyocytes exposed to H2O2.
Conclusion:
7-hydroxyflavone has potential as an effective antioxidant supplement for cardiac tissues. Further research is required to elucidate its pharmacokinetics and metabolic profile in humans to facilitate its therapeutic application.
