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Biosorption of Hexavalent Chromium by Shewanella putrefaciens MTCC 8104: Response Surface Methodology Optimization
Harsh Sable1, Vaishali Kumar1, Richa Mishra2
1Department of Forensic Science, Sharda School of Allied Health Sciences, Sharda University, Greater Noida, U.P. India.
Abstract:
Hexavalent chromium (Cr(VI)) is a widespread contaminant that poses an imminent danger to the well-being of humans. Recently, there has been a growing interest in using microbes to adsorb heavy metals. Nevertheless, research is limited on the process of biosorption of Cr(VI) by Shewanella putrefaciens MTCC 8104, a bacterium known for its ability to reduce metals. This article used single-factor experiments to examine the impact of hexavalent chromium on S. putrefaciens MTCC 8104. Additionally, response surface methodology (RSM) based on the Box-Behnken design (BBD) was employed to analyze the biosorption behavior of S. putrefaciens MTCC 8104 towards Cr(VI). The coefficient of determination (R2 = 6.35 for Cr(VI)) and probability value (p < 0.05) indicate relevance in the regression model. The model matched experimental data with S. putrefaciens MTCC 8104, achieving 86.50% Cr(VI) removal efficiency at 20 h contact time, pH 9, and biomass dosage of 12 g/L. Further experiments confirmed this result. A 3D response graph and ANOVA analysis revealed substantial impacts of temperature and the pH. The results of the experiments promoter that S. putrefaciens (MTTC 8104) might be a viable bacterial strain for recovery of Cr(VI) in polluted water.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s12088-024-01365-9.
