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Related Concept Videos

Hand hygiene01:23

Hand hygiene

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Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
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Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
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Chemical Agents for Microbial Control01:27

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Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
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Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

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Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
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Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
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Asepsis01:28

Asepsis

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The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
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Related Experiment Video

Updated: Jan 12, 2026

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection
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Persistent Pathogens Following Minimally Invasive Surgery: Is It Time to Rethink Aldehyde-Based Disinfection?

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  • 1Department of Digestive Diseases, Zen Multispeciality Hospital, Mumbai, IND.

Cureus
|November 3, 2025
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Summary
This summary is machine-generated.

Atypical mycobacterial infections are a growing concern after minimally invasive surgery. Aldehyde disinfection is linked to these infections, while autoclaving is protective, highlighting the need for better sterilization protocols.

Keywords:
atypical mycobacteriadeep mesh infectionminimal invasive gastrointestinal surgeryminimally invasive laparoscopyport site infection

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Area of Science:

  • Infectious Diseases
  • Surgical Site Infections
  • Microbiology

Background:

  • Atypical mycobacterial (ATM) infections pose a challenge in minimally invasive surgery (MIS), causing delayed surgical site infections (SSIs).
  • These infections are often difficult to diagnose and treat, impacting patient outcomes.
  • Understanding the link between sterilization practices and ATM infections is crucial for prevention.

Purpose of the Study:

  • To investigate the association between atypical mycobacterial infections and sterilization methods used in hospitals.
  • To evaluate the clinical presentations, outcomes, and prevention strategies for ATM infections following laparoscopic procedures.

Main Methods:

  • Retrospective review of 47 patients with delayed port-site complications after laparoscopic surgery.
  • Analysis of clinical presentations, microbiological data, and sterilization practices.
  • Correlation of ATM infections with specific disinfection methods like glutaraldehyde and autoclaving.

Main Results:

  • A strong association was found between ATM infections and the use of 2.45% glutaraldehyde without standardized protocols.
  • Autoclaving emerged as a protective sterilization method against ATM infections.
  • Common presentations included port-site sinuses, nodules, and abscesses, with infections caused by *Mycobacterium abscessus*, *M. fortuitum*, and the *M. avium* complex.

Conclusions:

  • Atypical mycobacterial infections are an under-recognized cause of post-laparoscopic SSIs.
  • Aldehyde-based disinfection is inadequate for preventing ATM infections; autoclaving is preferred.
  • Standardized sterilization protocols, improved early diagnosis, and optimized management guidelines are urgently needed.